Apart from their paucity, these recordings of MSNA in hypoxia are limited because they were made on fibres in the mixed nerve supply of the limb that were judged to be sympathetic and destined for skeletal muscle vasculature on the basis of their firing characteristics (see Janig, 1988); their actual, final destination could not be established. Further, little attempt was made to analyse activity in single nerve fibres discriminated from the multiunit activity. This is important, firstly because an increase in multiunit activity cannot differentiate between an increase in activity in individual fibres and recruitment of fibres, and secondly because the patterning of activity in individual fibres plays crucial roles in determining the relative importance of the sympathetic co-transmitters noradrenaline, ATP and neuropeptide Y (NPY) in the evoked vasoconstrictor response (Huidoboro-Toro & Donoso, 2004).
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